| Improving Quantitative Phase Imaging to Enhance Reliability and Broaden Applications |
- Universal application of an OBM/qOBM optical phase imaging device is made possible by the optimized SNR that identifies configurations with fewer limitations (e.g., conditions, tissue).
- The simulated SNR can identify non-intuitive geometries for optical phase imaging devices that previously may not have been identified by the arduous experimental optimization process.
- Optimized SNR when OBM/qOBM devices take in-vivo and in-situ measurements have been proven reliable across multiple types of tissue.
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8936 |
|
| Enhanced Diversifying Base Editors for Directed Evolution in S. cerevisiae |
- This invention presents an optimized and integrated CRISPR diversifying base editor for use in yeast and demonstrate its ability to rapidly improve the affinity of an antibody through yeast display.
- The innovation has enhanced the base editor mutation rate up has been increased to 27-fold by characterizing an improved key variant and by optimizing the structure of the CRISPR guide RNAs.
- The technology has attained a rate roughly 10-fold higher than state-of-art systems and facilitates ultra-rapid antibody diversification.
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9111 |
|
| Novel Repressor Proteins for Gene Regulation and CRISPRi |
- The new CRISPRi platform uses novel fusion proteins to specifically inhibit target genes of interest and overcome the off-target issues with existing CRISPR platforms already on the market.
- The technology can be used as a life science tool and/or for diagnostics and therapeutics.
- The platform aims to limit off-target editing, circumvent incomplete gene knockdown sgRNA sequence-dependent repression activity, and variable performance across human cancer cell lines.
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9106 |
|
| Enabling Rapid Detection of Label-Free RNA for Use in COVID-19 Testing |
- Earlier detection: Identifying the viral components themselves—rather than the body’s reaction to the viral presence (i.e., antibodies)—potentially improves early detection in asymptomatic subjects.
- Simpler process: Use of minimal reagents and fewer preparatory steps is designed to enable more efficient and facile sample preparation. Avoidance of “wash steps” aims to minimize target loss.
- Fewer disruptions: Avoiding the use of high-demand supplies (e.g., swabs) and reagents potentially reduces supply-chain and contamination issues.
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8669 |
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| Messenger Ribonucleic Acid (mRNA)–Based Opsin Expression Enables New and Safer Optogenetic Studies |
- Opsin expression using messenger RNA (mRNA) provides an alternative to viral vectors for use in optogenetic studies involving neurons as well as drug testing with cardiomyocytes.
- Expression with mRNAs has lower toxicity and is usually detectable within 2–24 hours rather than 2–3 weeks.
- The controllable and temporary nature of mRNA offers improved expression regulation based on the transfection amount.
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7534 |
|
| Microfluidic Device Enables Prediction of T-Cell Ability to Home and Engraft to Disease Tissues |
- Improve the homing capabilities of T cells to increase adoptive cell therapy treatment response and safety while decreasing off-target effects.
- Enhance tumor-infiltrating capabilities with engineered microfluidic devices that identify optimal subsets of cells.
- Optimize development pipelines by reducing the number of laborious in vivo studies, while also helping cell therapy companies ensure their products reach the desired tissue.
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9052 |
|
| Lymphatic System-Specific Lipid Nanoparticles |
- This platform technology improves current targeting to lymphatic tissues and specifically lymphatic endothelial cells.
- Provides superior tissue targeting and functional delivery of mRNA to lymphatics via loco-regional dermal or subcutaneous avenues.
- mRNA-based platforms offer a transient and less immunogenic method of delivery compared to protein therapeutics or adeno-associated virus-based platforms.
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9056 |
|
| Soft Wireless Sternal Patch for Detecting Systemic Vasoconstriction Using Photoplethysmography |
- A wireless, soft sternal patch includes integrated skin-like, photolithographically patterned electronics specifically tuned to continuously measure vasoconstriction from the sternum.
- In overnight trials, the device detected sleep apnea and hypopnea with 95% precision and 100% sensitivity compared to data professionally scored by licensed clinicians.
- Use of this patch on the chest could improve detection of key markers of numerous harmful health conditions.
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9170 |
|
| Targeted Single Cell Electroporation for Gene and Cell Therapy |
- The technology enables high efficiency delivery and high post-electroporation cell viability compared to current commercial electroporation systems.
- The technology performs in-flow single cell measurements integrated with electroporation unlike current commercial electroporation systems which have no single cell measurement capabilities.
- The technology enables automatic feedback control and real-time optimization of electroporation parameters at the single-cell level unlike current commercial electroporation systems which apply the same parameters to all cells in a batch at a bulk scale.
- The technology enables selected or targeted electroporation and delivery to selected cells or cell types from a mixture of cells unlike current commercial electroporation systems which require upstream or downstream cell sorting steps.
|
9086 |
|
| Lymphoid Tissue System Harnesses Ex Vivo Immune Reactions for Rapid Antibody Production and Immune Response Prediction |
- This immunocompetent system uniquely accounts for the unexpected activation of various immune cells to allow proper immune cell differentiation, recapitulating an effective immune response.
- The chemokine gradient is switchable, allowing the immune cell differentiation needed for an appropriate immune reaction.
- An ex vivo system supports immune response creation in a controlled environment, allowing reproduction of immunologic events for developing improved therapeutics.
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9196 |
|
| Super-resolution microscope for 3D cell and tissue imaging |
- The technology facilitates super-resolution cell and tissue imaging with multiple colors.
- The technology provides easy access to commonly adopted protocols, which has an expected wide usage.
- 3D multifocal scanning microscopy facilitates stationary, multi-focal excitation, thus achieving super-resolution microscopy.
|
9321 |
|
| Synthetically Generated, Realistic, and Controllable Human Bio-Signals from Participant-Free Data Collection |
- Synthetic data generation system replicates human body signals and uses a deep generative model to create synthetic SCG beats without human participants.
- Transformer-based generative model synthesizes SCG beats that are physiologically diverse and realistic.
- Clinically relevant feature inputs act as controls for aortic opening (AO) and aortic closing (AC) features of the generated SCG beat.
|
9081 |
|
| Microfluidic System Enables Scalable, High-Throughput Separation for High-Purity Human Induced Pluripotent Stem Cells (hiPSCs) |
- Simple, tunable high-throughput process separates hiPSCs quickly and efficiently according to the degree to which they adhere to a substrate inside the microfluidic device.
- Separation of intact cell colonies enables this simple system to avoid damaging and adversely affecting the pluripotency of the cells.
- The approach is fast, does not rely on labeling technologies, supports greatly enriched cells, and results in a cell survival rate greater than 80%.
|
5685 |
|
| Universal Transcriptional Programming in a Bacteroides Consortium Creates a Foundation to Advance Living Therapeutics |
- Consortium-based, universal transcriptional programming framework is a biotic decision-making technology using genetic circuit compression in a Bacteroides community.
- Compressed genetic circuit design shortens circuit-building time, offers a compact circuit footprint that allows more complexity, uses fewer resources, and reduces cell communication time.
- Has potential applications in living therapeutics, advanced biomanufacturing, and next-generation biosecurity.
|
9006 |
|
| Fast, Effective Thrombolytic Agent for Arterial Blood Clots |
- The disulfide dimer of the amino acid cystine, has the potential to be a highly efficient thrombolytic agent for treating dangerous arterial blood clots.
- During in vitro testing DiNAC dissolved thrombi in as quickly as 90 seconds and reduced the diameter and surface area of certain thrombi by 50–95 percent.
- DiNAC has the potential to treat multiple conditions associated with arterial thrombus formation and to mitigate life-threatening side effects.
|
8007 |
|
| Single Cell Metabolomics Assay |
- The technology makes it possible to identify single cell resolution of lipids and other small molecules in complex tissue types, as well as metabolism mapping specific to different cell types.
- The measurements found through this can be used to study metabolomic response to drug exposure.
- The technology can achieve submicron resolution of lipids and other small molecules at anywhere from 200-1000 depth slices, using spatially resolved metabolic profiling.
|
9023 |
|
| Measurement and Modeling System Using Cell Signaling Networks |
- The technology quickly identifies cell signaling pathways from a patient biopsy, allowing for personalized treatment approaches to be identified.
- Measurements can be used to study potential tumor response to drug treatment, through the use of xenografted cells from the patient.
- 30-100 potential protein pathways and interactions can be measured using a single sample through multiplexing of rapid immunofluorescence.
|
9022 |
|
| Saliva-Based Multiplexed Assay with 25x Higher Sensitivity to SARS-CoV-2 and Influenza A and B |
- New lateral flow assay device achieves enhanced sensitivity with the same sample concentration, significantly reducing the risk of false negatives.
- Tunable capillary flow allows sample/reagent to be concurrently delivered to multiple regions, enabling multiplexed and repetitive assays on a single device.
- Transforms a variety of biological assays and tests performed at clinical laboratories into single-use, disposable dipstick tests to be used at the point of care or home.
|
9149 |
|
| Patterned Implantable Devices for Improved Reconstruction of Tubular Tissue Passageway Defects |
- This innovation improves patterned airway stents for passageways or tubular tissues to potentially enable long-passageway reconstructions.
- Extrusion-based 3D bioprinting produces a patterned implantable device with higher mechanical strength without increasing the wall thickness of the device.
- Incorporating auxetic-patterning creates an implantable splinting device for supporting passageway defects in growing patients, such as infants or children.
|
8831 |
|
| Novel Hydrogels for Encapsulation, Vascularization, and Transplantation of Cells |
- This hydrolytically degradable poly(ethylene glycol) (PEG) hydrogel leverages ester linkages combined at various ratios with non-degradable macromers to enable tunable degradation kinetics.
- Potential applications include controlled release and delivery of drugs or proteins as well as cell encapsulation. It can also be used as a delivery vehicle and adhesive for cells in transplantation settings.
- These innovative hydrogels allow for rapid hydrolytic cleavage in vivo but remain stable in vitro for weeks at neural pH 7 to support regenerative medicine techniques.
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9019 |
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