Georgia Tech inventors have created a system and method for precise delivery of cargos, including DNA, RNA, proteins, peptide, organelles, functionalized nanoparticles, virus, CRISPR, and exosomes. Through an in vitro or in vivo delivery to a system, the method focuses on a network of individual cells or a multicellular tissue construct, which is stabilized on the substrate or flowing through open channels in a microfluidic system. This technology creates possibilities to apply and locally control the injection of the solubilized cargo into cells/tissue of the substrate or channel and is suitable for multiplexed, parallel processing.
- Novelty – use of an electrically-charged liquid beam of electrospray
- Improved control – control of action on the scale of single cell/single pores
- Diversity – arbitrary diverse set of cargo
- Applications – suitable for both in vivo and in vitro applications
- Improved delivery – selective and direct delivery of charged cargo
Laboratory and clinical applications
- Gene and drug delivery
- Therapeutic cell modification
- CRISPR delivery
- Cell imaging
- Biologics production
The ability to inject DNA into cells is critical to any genetic, molecular, biology, drug design and delivery, and pharmaceutical research and development work. There is a need for unprecedented control of action on the scale of single cell/single pore, and the ability to deliver a diverse set of cellular subcomponents to a biological system.