Requirements in super-resolution imaging

The development of super-resolution imaging has significantly revolutionized cell biology. Current modern fluorescence microscopy excels at live cell imaging, which requires fast speed, less photo toxicity, and a broad field of view. There is however a tradeoff between extreme spatial resolution in field of view, time resolution and computational load. 

3D-MSM for super-resolution imaging of cells

Innovators at Georgia Tech have developed a three-dimensional multifocal scanning microscopy, or 3D-MSM, for super-resolution imaging of cells and tissues with substantially reduced instrumental complexity. 3D-MSM harnesses the inherent 3D movement of specimens to facilitate stationary, multi-focal excitation, thus achieving super-resolution microscopy via a standard epi-fluorescence platform. 3D-MSM can not only be used for live cell imaging but has also been shown effective over a variety of biological samples and can be used with multiple fluorescent probes. 

Solution Advantages
  • Super-Resolution: combines structured illumination microscopy with confocal microscopy for superior cell and tissue imaging with multiple fluorescent colors.
  • Easy Access: Easily implemented with existing microscopes allowing for quick and wide application. 
  • Multi-Dimensional: 3D multifocal scanning microscopy facilitates stationary and multi-focal excitation, thus achieving super-resolution microscopy.
Potential Commercial Applications
  • Usage in research microscopy settings
  • Usage in clinical microscopy settings