Requirements in super-resolution imaging
The development of super-resolution imaging has significantly revolutionized cell biology. Current modern fluorescence microscopy excels at live cell imaging, which requires fast speed, less photo toxicity, and a broad field of view. There is however a tradeoff between extreme spatial resolution in field of view, time resolution and computational load.
3D-MSM for super-resolution imaging of cells
Innovators at Georgia Tech have developed a three-dimensional multifocal scanning microscopy, or 3D-MSM, for super-resolution imaging of cells and tissues with substantially reduced instrumental complexity. 3D-MSM harnesses the inherent 3D movement of specimens to facilitate stationary, multi-focal excitation, thus achieving super-resolution microscopy via a standard epi-fluorescence platform. 3D-MSM can not only be used for live cell imaging but has also been shown effective over a variety of biological samples and can be used with multiple fluorescent probes.
- Super-Resolution: combines structured illumination microscopy with confocal microscopy for superior cell and tissue imaging with multiple fluorescent colors.
- Easy Access: Easily implemented with existing microscopes allowing for quick and wide application.
- Multi-Dimensional: 3D multifocal scanning microscopy facilitates stationary and multi-focal excitation, thus achieving super-resolution microscopy.
- Usage in research microscopy settings
- Usage in clinical microscopy settings