Georgia Tech’s novel culturing system uses micro- and milli-fluidics to achieve a controlled and homogeneous environment that promotes high-quality, long-term organoid growth.
The system utilizes novel mechanisms to distribute media, trap and remove organoids, and discharge waste products. The device consists of loading, trapping, and base plates that are configured to suspend each organoid within a single well of an array. The trapping mechanism includes a channel that is large enough for fluid to enter yet prevents the organoid from escaping. The device continuously perfuses media and removes waste at a uniform rate via a gravity-driven flow, eliminating the need for two reservoirs per cell-culturing chamber. Flow control barriers prevent waste transference or formation of stagnant flow between adjacent organoids.
Organoids can be removed all at once or individually. The loading and trapping plates are separate parts, allowing for easier device manipulation and access to organoids.
- Reliable: Supports a uniform culturing environment, enabling the production of high-quality homogeneous organoids for precision drug testing
- Powerful: Permits highly-throughput, long-term organoid culturing at the milli-scale
- Efficient: Continuously perfuses media and removes waste with minimal effort by laboratory workers
- Convenient: Enables simple and efficient organoid loading and removal
- Drug discovery and testing for precision/personalized medicine
- Research for understanding human organ physiology and pathophysiology
Organoids are miniaturized and simplified 3D versions of organs and are useful for studying diseases and treatments in laboratories. Organoid culturing has become an important part of conducting biological and clinical research. However, the variability in culturing methods prevents homogenous production of organoids and hinders the reproducibility needed for scientific investigation.
To maximize benefits, it is critical that organoids are as homogeneous as possible to reduce variability and increase controllability. The culturing environment is the primary controlling factor relating to organoid homogeneity, yet existing practices rarely include controls for media delivery, waste removal, and organoid arrangement. Often, organoids are arranged randomly with no definite spacing or spatial guides, while media delivery and waste removal are laboriously performed with repeated pipetting and aspirating.
Georgia Tech’s system supports a uniform culturing environment, leading to high-quality and long-term organoid growth.